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precision plus ladder  (Bio-Rad)


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    Structured Review

    Bio-Rad precision plus ladder
    Precision Plus Ladder, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 3029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/precision+plus+protein+dual+color+standard+protein+ladder/Precision+Plus+Protein+Dual+Color+Standards/pmc13162090-99-13-19
    Average 97 stars, based on 3029 article reviews
    precision plus ladder - by Bioz Stars, 2026-09
    97/100 stars

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    Nucleic Acid Electrophoresis:

    Article Title: Polymeric Nanoparticles with a Sera-Derived Coating for Efficient Cancer Cell Uptake and Killing
    Article Snippet: Paclitaxel (taxol equivalent) was obtained Thermofisher Scientific. .. For gel electrophoresis, Mini-PROTEAN TGX polyacrylamide gels and Precision Plus protein dual-color standard protein ladder were purchased from Bio-Rad Laboratories (Hercules, CA, USA). β-Mercaptoethanol was obtained from Millipore Sigma. .. Coomassie Brilliant Blue was purchased from ThermoFisher Scientific.

    Article Title: Polymeric Nanoparticles with a Sera-Derived Coating for Efficient Cancer Cell Uptake and Killing.
    Article Snippet: Paclitaxel (taxol equivalent) was obtained Thermofisher Scientific. .. For gel electrophoresis, Mini-PROTEAN TGX polyacrylamide gels and Precision Plus protein dual-color standard protein ladder were purchased from Bio-Rad Laboratories (Hercules, CA, USA). β-Mercaptoethanol was obtained from Millipore Sigma. .. Coomassie Brilliant Blue was purchased from ThermoFisher Scientific.

    Electrophoresis:

    Article Title: Acute stress enhances heterodimerization and binding of corticosteroid receptors at glucocorticoid target genes in the hippocampus
    Article Snippet: Protein was quantified using the Qubit Protein Assay Kit (Life Technologies) as per the manufacturer’s instructions using a Qubit 2.0. .. Nuclear protein samples (20 μg per well) and Precision Plus Protein Dual Color Standard Protein Ladder (BioRad) were loaded onto 4–15% (wt/vol) acrylamide Mini-PROTEAN TGX Precast Gels (BioRad) and separated by electrophoresis for ∼45 min at 200 V. Proteins were transferred to a PVDF membrane (BioRad) and blocked overnight [GR: 5% (wt/vol) BSA/PBS-T; MR: 5% (wt/vol) milk/phosphate buffered saline/Tween-20 (PBS-T)] at 4 °C. .. Membranes were incubated with either anti-GR (1:500; H-300; sc-8992X; Santa Cruz) or anti-MR (1:500; H-300; sc-11412X; Santa Cruz) in 1% blocking solution for 2 h at room temperature.

    Membrane:

    Article Title: Acute stress enhances heterodimerization and binding of corticosteroid receptors at glucocorticoid target genes in the hippocampus
    Article Snippet: Protein was quantified using the Qubit Protein Assay Kit (Life Technologies) as per the manufacturer’s instructions using a Qubit 2.0. .. Nuclear protein samples (20 μg per well) and Precision Plus Protein Dual Color Standard Protein Ladder (BioRad) were loaded onto 4–15% (wt/vol) acrylamide Mini-PROTEAN TGX Precast Gels (BioRad) and separated by electrophoresis for ∼45 min at 200 V. Proteins were transferred to a PVDF membrane (BioRad) and blocked overnight [GR: 5% (wt/vol) BSA/PBS-T; MR: 5% (wt/vol) milk/phosphate buffered saline/Tween-20 (PBS-T)] at 4 °C. .. Membranes were incubated with either anti-GR (1:500; H-300; sc-8992X; Santa Cruz) or anti-MR (1:500; H-300; sc-11412X; Santa Cruz) in 1% blocking solution for 2 h at room temperature.

    Saline:

    Article Title: Acute stress enhances heterodimerization and binding of corticosteroid receptors at glucocorticoid target genes in the hippocampus
    Article Snippet: Protein was quantified using the Qubit Protein Assay Kit (Life Technologies) as per the manufacturer’s instructions using a Qubit 2.0. .. Nuclear protein samples (20 μg per well) and Precision Plus Protein Dual Color Standard Protein Ladder (BioRad) were loaded onto 4–15% (wt/vol) acrylamide Mini-PROTEAN TGX Precast Gels (BioRad) and separated by electrophoresis for ∼45 min at 200 V. Proteins were transferred to a PVDF membrane (BioRad) and blocked overnight [GR: 5% (wt/vol) BSA/PBS-T; MR: 5% (wt/vol) milk/phosphate buffered saline/Tween-20 (PBS-T)] at 4 °C. .. Membranes were incubated with either anti-GR (1:500; H-300; sc-8992X; Santa Cruz) or anti-MR (1:500; H-300; sc-11412X; Santa Cruz) in 1% blocking solution for 2 h at room temperature.



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